Next Generation Sequencing Courses

Beginners Courses in Next Generation Sequencing

Although originally developed for whole genome sequencing, next generation sequencing is now increasingly used in other areas including measurement of mRNAs, miRNAs and other long non-coding RNAs (RNASeq), DNA-protein interactions (ChIPSeq) and exon/DNA sequencing (DNASeq). This technique is revolutionising our understanding of biology and is predicted to lay the path for the development of personalised medicine. Furthermore, the rapid increase in sequencing capacity, reduction in costs and the proliferation of sequencing companies has bought this technology within reach of most scientists and clinicians. At NextGenSeq we aim to open up this powerful technology to scientists and clinicians with little or no previous experience by organising small intensive courses in various aspects of next generation sequencing.

Measurement and analysis of mRNAs, long non-coding RNAs and microRNAs using next generation sequencing (RNAseq)

Aims: The rapid reductions in the price of sequencing has meant that RNA sequencing (RNAseq) is replacing microarrays as the option of choice for measuring the transcriptome. This one day course is designed for scientists and clinicians with little or no experience in RNASeq. The course aims to provide the experimental and bioinformatics skills required to prepare samples, quantify the levels of known/novel mRNAs, long intergenic non-coding RNAs and miRNAs using next generation sequencing data. We assume that sequencing will be performed by an external provider and will provide advice in this area. The course is computer based and will involve a combination of presentations/exercises to analyse 'actual' next generation sequencing data using publically available programmes. This course is designed to complement the ChIPseq course.

Dates and location:

Thursday 21st October 2021 - On-line course (Maximum 8 people)

Tuesday 14th December 2021 - On-line course (Maximum 8 people)

Cost : £249

For reservations contact mailto:m.a.lindsay@bath.ac.uk

Further information and payment at rnaseq.php

Measurement and analysis of DNA-Protein interactions using chromatin immunoprecipitation and next generation sequencing (ChIPseq)

Aims: Epigenetics is emerging as an important regulatory mechanism and central to understanding this process is the ability to measure protein-DNA interactions including the effect of histones modifications and transcription factor binding (ChIPseq). This one day course is designed for scientists and clinicians with little or no experience in ChIPseq and is based upon the recommended protocols outlined in the ENCODE project. The course aims to provide the experimental and bioinformatics skills required to prepare samples, quantify the levels of protein-DNA binding (transcription factors and histones) using next generation sequencing and examine the overlap between ChIPseq and RNA expression data (see RNAseq course). We assume that sequencing will be performed by an external provider and will provide advice in this area. The course is computer based and will involve a combination of presentations/exercises to analyse 'actual' next generation sequencing data using publically available programmes. This course is designed to complement the RNAseq.

Dates and Locations:

Wednesday 15th December 2021 - On-line course (maximum 8 people)

Cost: £249

For reservations contact mailto:m.a.lindsay@bath.ac.uk

Further information and payment at chipseq.php

Exome/DNA analysis using next generation sequencing (DNASeq)

Aims: With the wealth of data being generated by the 100,000 Genomes Project and other databases, it is now becoming increasing important to understand the process by which these results are generated and interpreted. This one day course is designed for scientists and clinicians with little or no experience in exome/DNA sequencing. The course is based upon the protocol described by the 100,000 genome project and aims to provide the experimental and bioinformatics skills required to prepare samples, undertake exome sequencing and the subsequent identification of single nucleotide variations (SNVs) and short Insertions/Deletions (Indels). The course will provide information on the interpretation of this sequencing data including disease association and pathway/GO analysis. We assume that sequencing will be performed by an external provider and will provide advice in this area. The course is computer based and will involve a combination of presentations/exercises to analyse 'actual' next generation sequencing data using publically available programmes.

Dates and location:

Friday 19th November - On-line course (Maximum 8 people)

Cost: £249

For reservations contact mailto:m.a.lindsay@bath.ac.uk

Further information and payment at dnaseq.php

Booking

For further information and/or to reserve a place contact Mark Lindsay on mailto:m.a.lindsay@bath.ac.uk

Payment

Credit and Debit Card payments: see individual pages

By cheque: Send a cheque made payable to NextGenSeq Limited to 40 Wingfield Road, Trowbridge, Wiltshire, BA14 9ED

BACS transfer: NextGenSeq Limited, HSBC, Sort Code: 40-44-33, Account Code: 72038145

Purchase Order: Please send PO to NextGenSeq Limited, 40 Wingfield Road, Trowbridge, Wiltshire, BA14 9ED

Cancellation Policy: Greater than 7 days (100% refund) and less than 7 days (50% refund)